A landmark protein essential for establishing and perpetuating the polarity of a bacterial cell

被引：145
作者：
Lam, H [1 ]
Schofield, WB [1 ]
Jacobs-Wagner, C [1 ]
机构：
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
来源：
CELL | 2006年 / 124卷 / 05期
关键词：
D O I：
10.1016/j.cell.2005.12.040
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polarity is often an intrinsic property of the cell, yet little is known about its origin or its maintenance over generations. Here we identify a landmark protein, TipN, which acts as a spatial and temporal cue for setting up the correct polarity in the bacterium Caulobacter crescentus. TipN marks the new pole throughout most of the cell cycle, and its relocation to the nascent poles at the end of division provides a preexisting reference point for orienting the polarity axis in the progeny. Deletion of tipN causes pleiotropic polarity defects, including frequently reversed asymmetry in progeny size and mislocalization of proteins and organelles. Ectopic localization of TipN along the lateral side of the cell creates new axes of polarity leading to cell branching and formation of competent cell poles. Localization defects of the actin-like protein MreB in the Delta tipN mutant suggest that TipN is upstream of MreB in regulating cell polarity.
引用
收藏
页码：1011 / 1023
页数：13
相关论文
共 31 条
  • [11] MreB actin-mediated segregation of a specific region of a bacterial chromosome
    Gital, Z
    Dye, NA
    Reisenauer, A
    Wachi, M
    Shapiro, L
    [J]. CELL, 2005, 120 (03) : 329 - 341
  • [12] Physiological consequences of blocked Caulobacter crescentus dnaA expression, an essential DNA replication gene
    Gorbatyuk, B
    Marczynski, GT
    [J]. MOLECULAR MICROBIOLOGY, 2001, 40 (02) : 485 - 497
  • [13] Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division
    Jacobs, C
    Domian, IJ
    Maddock, JR
    Shapiro, L
    [J]. CELL, 1999, 97 (01) : 111 - 120
  • [14] Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in Caulobacter
    Judd, EM
    Ryan, KR
    Moerner, WE
    Shapiro, L
    McAdams, HH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (14) : 8235 - 8240
  • [15] Lupas A, 1996, METHOD ENZYMOL, V266, P513
  • [16] Cytokinesis monitoring during development:: Rapid pole-to-pole shuttling of a signaling protein by localized kinase and phosphatase in Caulobacter
    Matroule, JY
    Lam, H
    Burnette, DT
    Jacobs-Wagner, C
    [J]. CELL, 2004, 118 (05) : 579 - 590
  • [17] NIEMAN WC, 2001, P NATL ACAD SCI USA, V98, P4136
  • [18] Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB
    Nilsen, T
    Yan, AW
    Gale, G
    Goldberg, MB
    [J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (17) : 6187 - 6196
  • [19] Signal transduction in the cell cycle regulation of Caulobacter differentiation
    Ohta, N
    Newton, A
    [J]. TRENDS IN MICROBIOLOGY, 1996, 4 (08) : 326 - 332
  • [20] Identification of a Caulobacter crescentus operon encoding hrcA, involved in negatively regulating heat-inducible transcription, and the chaperone gene grpE
    Roberts, RC
    Toochinda, C
    Avedissian, M
    Baldini, RL
    Gomes, SL
    Shapiro, L
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (07) : 1829 - 1841
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