Preparation and biosensing performance of porous-alumina-assisted gold nanostructures on substrates

Gold based nanosize structures such as nanoparticles and nanowires are considered as important building blocks for nanotechnology to be used for construction of sensing micro- and nanodevices because of their excellent optical, electrical and mechanical properties. Here we synthesize via porous-alumina-assisted anodizing and electrodeposition perfect arrays of gold nanodots and nanorods, upright standing, spatially separated and highly aligned on a conductive substrate, which are expected to have exceptional performance in nanoscale electronic, optoelectronics and sensing applications. Biosensing potential of the nanostructured electrodes was assessed by examining electrochemical properties of a protein layer grafted on the electrode for Creactive protein (CRP) detection using CRP-antibody immobilization method with protein G intermediate layer. The laboratory prototype of immunosensor revealed reproducible, rapid and stable response with a low limit for CRP-antigen detection of 100 fg . ml(-1) (C) 2016 The Authors. Published by Elsevier Ltd.