ATP-dependent H+-pump activity in inverted vesicles of Methanosarcina mazei Go1 and characterization of membrane ATPase

被引:5
|
作者
Inatomi, K
机构
[1] Advanced Technology R and D Center, Mitsubishi Electric Corporation, Hyogo 661, 8-1-1, Tsukaguchi Amagasaki
关键词
D O I
10.1128/jb.178.8.2424-2426.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
ATP-dependent H+-pump activity was found in inverted vesicles of Methanosarcina mazei Go1 by using acridine orange as a fluorescent probe. The H+-pump activity specifically required both Mg and sulfite ions, but azide, an inhibitor of F0F1-ATPase, did not inhibit the activity. The membranes prepared from M. mazei also had an Mg-ATPase activity, and at least the presence of vacuolar-type ATPase was detected.
引用
收藏
页码:2424 / 2426
页数:3
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