De Novo Transcriptome Assembly (NGS) of Curcuma longa L. Rhizome Reveals Novel Transcripts Related to Anticancer and Antimalarial Terpenoids

被引:59
作者
Annadurai, Ramasamy S. [1 ]
Neethiraj, Ramprasad [1 ]
Jayakumar, Vasanthan [1 ]
Damodaran, Anand C. [1 ]
Rao, Sudha Narayana [1 ]
Katta, Mohan A. V. S. K. [1 ]
Gopinathan, Sreeja [1 ]
Sarma, Santosh Prasad [1 ]
Senthilkumar, Vanitha [1 ]
Niranjan, Vidya [1 ]
Gopinath, Ashok [1 ]
Mugasimangalam, Raja C. [1 ]
机构
[1] Genotyp Technol Private Ltd, Res & Dev Unit, Bangalore, Karnataka, India
来源
PLOS ONE | 2013年 / 8卷 / 02期
关键词
CELL-CYCLE; MULTIDRUG-RESISTANCE; FRUIT-DEVELOPMENT; ESSENTIAL OILS; APOPTOSIS; HYPERICIN; DISCOVERY; PATHWAYS; MARKERS; GENES;
D O I
10.1371/journal.pone.0056217
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.
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页数:11
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