Light quality and growth regulators on in vitro multiplication and rooting of blackberry 'Xavante'

There are a lot of advantages using micropropagation technique over the methods traditionally used in blackberry propagation especially concerning health of seedlings and speed in getting new plants. The aim of this study was to investigate the influence of bioregulators 6-benzylaminopurine (BAP) and indol-3-butyric acid (IBA) combined with light quality on in vitro multiplication and rooting, respectively, of blackberry 'Xavante'. The experimental design was completely randomized. The treatments consisted of different levels of light quality (blue, red, white and absence of light) combined with the absence or presence of bioregulators IBA (0.5mg L-1) and BAP (0.8mg L-1) in the culture medium. The use of BAP increases the number of new shoots, buds and leaves of blackberry, 'Xavante' and thus is suitable for its in vitro propagation. The use of IBA is not required in in vitro rooting of the blackberry cultivar studied, showing that it is naturally well rooting.