A thermostable α-amylase producing maltohexaose from a new isolated Bacillus sp US100:: study of activity and molecular cloning of the corresponding gene

被引：0
作者：
Ben Ali, M [1 ]
Mezghani, M [1 ]
Bejar, S [1 ]
机构：
[1] Ctr Biotechnol Sfax, Sfax 3038, Tunisia
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1999年 / 24卷 / 8-9期
关键词：
alpha-amylase; B-stearothermophilus; thermoamylase; maltohexaose maltopentaose; molecular cloning;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Bacillus sp. US100 strain was isolated from Trunisian hot spring soil. This strain secretes a thermoactive amylase having an optimal temperature of 82 degrees C and a remarkable thermostability with a half time of 40 min at 110 degrees C in the presence of 20% (w/v) of substrate. The main end products of its action an starch are maltohexaose and maltopentaose which is the smallest substrate hydrolyzed by this enzyme. The molecular cloning, in E. coli, of a 3-kb DNA fragment encoding far this activity is also reported (C) 1999 Elsevier Science Inc. All rights reserved.
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页码：584 / 589
页数：6
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