Image formation in structured illumination wide-field fluorescence microscopy

被引：99

作者：

Karadaglic, Dejan ^{[1
]}

Wilson, Tony ^{[2
]}

机构：

[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England

[2] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

MICRON | 2008年 / 39卷 / 07期

关键词：

structured illumination; optical sectioning; optical microscopy; fluorescence microscopy; wide-field microscopy; confocal microscopy;

D O I：

10.1016/j.micron.2008.01.017

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：808 / 818

页数：11

共 18 条

[1]

[Anonymous], 1990, CONFOCAL MICROSCOPY

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