Antibacterial mechanism of Myagropsis myagroides extract on Listeria monocytogenes

被引：47

作者：

Lee, So-Young ^{[1
]}

Kim, Koth-Bong-Woo-Ri ^{[2
]}

Lim, Seong-Il ^{[1
]}

Ahn, Dong-Hyun ^{[2
]}

机构：

[1] Korea Food Res Inst, Fermentat & Funct Res Grp, Songnam 463746, South Korea

[2] Pukyong Natl Univ, Inst Food Sci, Dept Food Sci & Technol, Pusan 608737, South Korea

来源：

FOOD CONTROL | 2014年 / 42卷

关键词：

Antimicrobial action; Algae; Listeria monocytogenes; Cytoplasmic membrane damage; ATP leakage; TEM; BROWN ALGA; ESSENTIAL OILS; ECKLONIA-STOLONIFERA; MERODITERPENOIDS; PLASTOQUINONES; ANTIOXIDANT; DERIVATIVES; SPOILAGE;

D O I：

10.1016/j.foodcont.2014.01.030

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The ethanol extract of Myagropsis myagroides had antimicrobial activity against Gram-positive bacteria. The extract was fractionated through liquid-liquid extraction; the chloroform fraction had strong antimicrobial activity against Listeria monocytogenes (minimum inhibitory concentration (MIC) 0.063 mg/mL), and Clostridium perfringens (MIC 0.031 mg/mL). The chloroform fraction was separated into 22 sub-fractions using silica gel column chromatography, with the fourth fraction (CH4) possessing the strongest antimicrobial activity against Gram-positive bacteria. Leakage of 260 nm-absorbing material and ATP was observed in CH4-treated cells and morphological alterations were observed by electron microscopy. These results indicate that the cytoplasmic membrane may be a target of the CH4 fraction. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：23 / 28

页数：6

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