Preclinical evaluation of magainin-A as a contraceptive antimicrobial agent

被引：21

作者：

Clara, A ^{[1
]}

Manjramkar, DD ^{[1
]}

Reddy, VK ^{[1
]}

机构：

[1] Indian Council Med Res, Natl Inst Res Reprod Hlth, Immunol Lab, Bombay 400012, Maharashtra, India

来源：

FERTILITY AND STERILITY | 2004年 / 81卷 / 05期

关键词：

sperm motility; spermicidal activity; microbicide; vaginal contraceptive;

D O I：

10.1016/j.fertnstert.2003.09.073

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To evaluate the safety and contraceptive efficacy of magainin-A in monkeys. Design: Controlled laboratory study. Setting: Department of Immunology, National Institute for Research in Reproductive Health, Parel, Mumbai, India. Animal(s): Male and female bonnet monkeys (Macaca radiata). Intervention(s): Animals were treated intravaginally with 1 mg of magainin-A before attempted conception, as well as daily for 14 days to assess local and systemic toxicity. Main Outcome Measure(s): Suitability of magainin-A for the control of pregnancy and sexually transmitted infections. Result(s): Complete sperm immobilization was observed within 20 seconds after the exposure to magainin-A (800-1,000 mug) in vitro. Intravaginal administration of 1 mg of magainin-A blocked conception in monkeys. When magainin-A was administered intravaginally for 14 consecutive days, no treatment-related abnormalities were observed in menstrual cycle length, vaginal epithelial cell morphology, and hematologic/serum biochemical profiles. The peptide inhibited the growth of sexually transmitted infection-causing pathogens but not HIV-1 and HIV-2. Conclusion(s): Magainin-A can be used as an effective and safe intravaginal contraceptive compound with additional protection against sexually transmitted infection-causing pathogens.

引用

页码：1357 / 1365

页数：9

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