Mutations in the VP2 gene of rotavirus associated with benzimidazole sensitivity

被引：1

作者：

Chavez-Maya, Fernando ^{[1
]}

Garcia-Espinosa, Gary ^{[1
]}

Eugenia Lopez-Arellano, Maria ^{[2
]}

Padilla-Noriega, Luis ^{[3
]}

机构：

[1] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Med & Zootecnia Aves, Ciudad De Mexico 04510, Mexico

[2] INIFAP Mexico, Dept Helmintol, Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, Jiutepec 62550, Morelos, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol, Ciudad Univ,Edificio A, Ciudad De Mexico 04510, Mexico

来源：

VIRUS RESEARCH | 2021年 / 291卷

关键词：

Rotavirus; Mutant proteins; VP2; protein; RNA replicase; Benzimidazole; BOVINE ROTAVIRUS; PROTEIN; MORPHOGENESIS; REPLICATION; INFECTION; RECOGNITION; REQUIRES; DOMAIN; NSP5;

D O I：

10.1016/j.virusres.2020.198189

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rotavirus species A (RVA) is the etiological agent of acute gastroenteritis in young individuals of various animal species, including humans. Vaccination has helped to reduce the impact of these viruses on humans and some species of domestic mammals, but they do not confer complete immunity, so antirotavirus agents are another important control option. In this study, millimolar concentrations of benzimidazole inhibited the replication of the Rhesus rotavirus (RRV) strain of RVA. Two mutants partially resistant to the inhibitory effect of benzimidazole were independently selected, and their genomes and those of their parental strains were fully sequenced. Most (7/11) mutations occurred in the gene that encodes the VP2 protein, and similarly most of the missense mutations (5/9), including the only one shared by the two mutants (G2,414 -> R[G/A], D800 N), occurred in the VP2 gene. Our results identify the VP2 gene as the primary target affected by benzimidazole.

引用

页数：8

共 49 条

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