Comparison of Two PCR Assays for Trichomonas vaginalis

被引：5

作者：

Noh, Chang-Suk ^{[1
]}

Kim, Sang-Su ^{[2
,3
]}

Park, Sung-Yul ^{[4
]}

Moon, Hong-Sang ^{[4
]}

Hong, Yeonchul ^{[5
]}

Ryu, Jae-Sook ^{[2
,3
]}

机构：

[1] Hallym Univ, Hangang Sacred Heart Hosp, Dept Internal Med, Seoul 07247, South Korea

[2] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Environm Biol & Med Parasitol, Seoul, South Korea

[3] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Biomed Sci, Seoul, South Korea

[4] Hanyang Univ, Coll Med, Dept Urol, Seoul 04763, South Korea

[5] Kyungpook Natl Univ, Sch Med, Dept Parasitol & Trop Med, Daegu 41944, South Korea

来源：

KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Trichomonas vaginalis; PCR; DNA isolation; INFECTION; DIAGNOSIS; HIV-1; DNA;

D O I：

10.3347/kjp.2019.57.1.27

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit (R), using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

引用

页码：27 / 31

页数：5

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