Comparison of Two PCR Assays for Trichomonas vaginalis

被引：5
|
作者：
Noh, Chang-Suk [1 ]
Kim, Sang-Su [2 ,3 ]
Park, Sung-Yul [4 ]
Moon, Hong-Sang [4 ]
Hong, Yeonchul [5 ]
Ryu, Jae-Sook [2 ,3 ]
机构：
[1] Hallym Univ, Hangang Sacred Heart Hosp, Dept Internal Med, Seoul 07247, South Korea
[2] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Environm Biol & Med Parasitol, Seoul, South Korea
[3] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Biomed Sci, Seoul, South Korea
[4] Hanyang Univ, Coll Med, Dept Urol, Seoul 04763, South Korea
[5] Kyungpook Natl Univ, Sch Med, Dept Parasitol & Trop Med, Daegu 41944, South Korea
来源：
KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
Trichomonas vaginalis; PCR; DNA isolation; INFECTION; DIAGNOSIS; HIV-1; DNA;
D O I：
10.3347/kjp.2019.57.1.27
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit (R), using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
引用
收藏
页码：27 / 31
页数：5
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