Comparison of Two PCR Assays for Trichomonas vaginalis

被引：5

作者：

Noh, Chang-Suk ^{[1
]}

Kim, Sang-Su ^{[2
,3
]}

Park, Sung-Yul ^{[4
]}

Moon, Hong-Sang ^{[4
]}

Hong, Yeonchul ^{[5
]}

Ryu, Jae-Sook ^{[2
,3
]}

机构：

[1] Hallym Univ, Hangang Sacred Heart Hosp, Dept Internal Med, Seoul 07247, South Korea

[2] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Environm Biol & Med Parasitol, Seoul, South Korea

[3] Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Biomed Sci, Seoul, South Korea

[4] Hanyang Univ, Coll Med, Dept Urol, Seoul 04763, South Korea

[5] Kyungpook Natl Univ, Sch Med, Dept Parasitol & Trop Med, Daegu 41944, South Korea

来源：

KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Trichomonas vaginalis; PCR; DNA isolation; INFECTION; DIAGNOSIS; HIV-1; DNA;

D O I：

10.3347/kjp.2019.57.1.27

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit (R), using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

引用

页码：27 / 31

页数：5

共 16 条

[1] Strategies for Prevention and Treatment of Trichomonas vaginalis Infections
Bouchemal, Kawthar
Bories, Christian
Loiseau, Philippe M.
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2017, 30 (03) : 811 - 825
[2] Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis
Dierkes, Christine
Ehrenstein, Boris
Siebig, Sylvia
Linde, Hans-Joerg
Reischl, Udo
Salzberger, Bernd
[J]. BMC INFECTIOUS DISEASES, 2009, 9 : 126
[3] Impact of T. vaginalis infection on innate immune responses and reproductive outcome
Fichorova, Raina N.
[J]. JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2009, 83 (1-2) : 185 - 189
[4] CELL-CULTURE COMPARED WITH BROTH FOR DETECTION OF TRICHOMONAS-VAGINALIS
GARBER, GE
SIBAU, L
MA, R
PROCTOR, EM
SHAW, CE
BOWIE, WR
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (07) : 1275 - 1279
[5] Trichomonas vaginalis-induced epithelial monolayer disruption and human immunodeficiency virus type 1 (HIV-1) replication:: Implications for the sexual transmission of HIV-1
Guenthner, PC
Secor, WE
Dezzutti, CS
[J]. INFECTION AND IMMUNITY, 2005, 73 (07) : 4155 - 4160
[6] Validation of a urine-based PCR-enzyme-linked immunosorbent assay for use in clinical research settings to detect Trichomonas vaginalis in men
Kaydos-Daniels, SC
Miller, WC
Hoffman, I
Banda, T
Dzinyemba, W
Martinson, F
Cohen, MS
Hobbs, MM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (01) : 318 - 323
[7] PCR for Diagnosis of Male Trichomonas vaginalis Infection with Chronic Prostatitis and Urethritis
Lee, Jong Jin
Moon, Hong Sang
Lee, Tchun Yong
Hwang, Hwan Sik
Ahn, Myoung-Hee
Ryu, Jae-Sook
[J]. KOREAN JOURNAL OF PARASITOLOGY, 2012, 50 (02) : 157 - 159
[8] Evaluation of Seeplex® STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections
Lee, Seung-Ju
Park, Dong Choon
Lee, Dong Sup
Choe, Hyun-Sop
Cho, Yong-Hyun
[J]. JOURNAL OF INFECTION AND CHEMOTHERAPY, 2012, 18 (04) : 494 - 500
[9] Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples
Madico, G
Quinn, TC
Rompalo, A
McKee, KT
Gaydos, CA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) : 3205 - 3210
[10] Trichomonas vaginalis: Pathogenesis, Symbiont Interactions, and Host Cell Immune Responses
Mercer, Frances
Johnson, Patricia J.
[J]. TRENDS IN PARASITOLOGY, 2018, 34 (08) : 683 - 693

← 1 2 →