A mixed hemadsorption assay for detection of cell surface binding anti-tumor antibodies in human sera

被引：2

作者：

Akis, N ^{[1
]}

Andl, C ^{[1
]}

Zhou, DW ^{[1
]}

机构：

[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 261卷 / 1-2期

关键词：

mixed hemadsorption assay; GA733; humoral response in cancer;

D O I：

10.1016/S0022-1759(01)00560-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A practical mixed hemadsorption assay (MHA) was developed for the detection of cell surface binding anti-tumor antigen antibodies (anti-TA Ab) in the human sera. The assay was compared to enzyme-linked immunosorbent assay (ELISA) and dot ELISA in the detection of the antibodies developed against GA733-2 antigen. SW1116 cell line and recombinant GA733-2E protein were used as targets in the MHA and solid phase immunoassays (SPIA), respectively. Sera were obtained from healthy donors and vaccinated-colon carcinoma patients. The sensitivity level of the MHA was very high for detection of anti-TA Ab in sera. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 127

页数：9