Bacterial identification from blood cultures by a rapid Matrix-Assisted Laser Desorption-Ionisation Time-of-Flight mass spectrometry technique

被引:8
作者
Hoyos-Mallecot, Yanick [1 ]
Miranda-Casas, Consuelo [1 ]
Cabrera-Alvargonzalez, Jorge J. [1 ]
Gomez-Camarasa, Cristina [1 ]
Dolores Perez-Ramirez, M. [1 ]
Maria Navarro-Mari, Jose [1 ]
机构
[1] Hosp Virgen de las Nieves, Microbiol Serv, Granada, Spain
来源
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2013年 / 31卷 / 03期
关键词
Mass spectrometry; Matrix-assisted laser desorption-ionisation time-of-flight; Bacteria identification; Blood culture;
D O I
10.1016/j.eimc.2012.09.003
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Introduction: Matrix-Assisted Laser Desorption-Ionisation Time-of-Flight (MALDI-TOF) Mass Spectrometry is rapid and accurate for the bacterial identification. Methods: We have evaluated a less laborious and less time consuming method for microorganism identification directly from positive blood cultures. Results: When we considered the scores >= 1.7 and >= 1.4 for acceptable identification of species and genus, the percentage of identification was 77.5% and 93.9%, respectively. Conclusions: This method is reliable, rapid and cost-effective for implementation in routine use in clinical microbiology laboratories. (C) 2012 Elsevier Espana, S.L. All rights reserved.
引用
收藏
页码:152 / 155
页数:4
相关论文
共 10 条
[1]   Comparison of Direct Colony Method versus Extraction Method for Identification of Gram-Positive Cocci by Use of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].
Alatoom, Adnan A. ;
Cunningham, Scott A. ;
Ihde, Sherry M. ;
Mandrekar, Jayawant ;
Patel, Robin .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) :2868-2873
[2]  
Bille E, 2011, CLIN MICROBIOL INFEC, P27
[3]   Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].
Ferreira, L. ;
Sanchez-Juanes, F. ;
Porras-Guerra, I. ;
Garcia-Garcia, M. I. ;
Garcia-Sanchez, J. E. ;
Gonzalez-Buitrago, J. M. ;
Munoz-Bellido, J. L. .
CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (04) :546-551
[4]   Real-Time Identification of Bacteria and Candida Species in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].
Ferroni, Agnes ;
Suarez, Stephanie ;
Beretti, Jean-Luc ;
Dauphin, Brunhilde ;
Bille, Emmanuelle ;
Meyer, Julie ;
Bougnoux, Marie-Elisabeth ;
Alanio, Alexandre ;
Berche, Patrick ;
Nassif, Xavier .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (05) :1542-1548
[5]   Misidentification of alpha-hemolytic streptococci by routine tests in clinical practice [J].
Ikryannikova, L. N. ;
Lapin, K. N. ;
Malakhova, M. V. ;
Filimonova, A. V. ;
Ilina, E. N. ;
Dubovickaya, V. A. ;
Sidorenko, S. V. ;
Govorun, V. M. .
INFECTION GENETICS AND EVOLUTION, 2011, 11 (07) :1709-1715
[6]   Identification of Bacteria in Blood Culture Broths Using Matrix-Assisted Laser Desorption-Ionization Sepsityper™ and Time of Flight Mass Spectrometry [J].
Kok, Jen ;
Thomas, Lee C. ;
Olma, Thomas ;
Chen, Sharon C. A. ;
Iredell, Jonathan R. .
PLOS ONE, 2011, 6 (08)
[7]  
Martiny D, 2012, EUR J CLIN MICROBIOL, P25
[8]   Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identifies 90% of bacteria directly from blood culture vials [J].
Moussaoui, W. ;
Jaulhac, B. ;
Hoffmann, A. -M. ;
Kostrzewa, M. ;
Riegel, P. ;
Prevost, G. .
CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (11) :1631-1638
[9]   Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry [J].
Saffert, Ryan T. ;
Cunningham, Scott A. ;
Mandrekar, Jayawant ;
Patel, Robin .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 73 (01) :21-26
[10]   Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) [J].
Van Herendael, B. H. ;
Bruynseels, P. ;
Bensaid, M. ;
Boekhout, T. ;
De Baere, T. ;
Surmont, I. ;
Mertens, A. H. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (05) :841-848