Evaluation of corncob hemicellulosic hydrolysate for xylitol production by adapted strain of Candida tropicalis

被引:68
作者
Misra, Swati [1 ]
Raghuwanshi, Shailendra [1 ]
Saxena, R. K. [1 ]
机构
[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India
关键词
Xylose; Corncob hemicellulosic hydrolysate; C; tropicalis; Xylitol; Immobilization; D-XYLOSE; DEBARYOMYCES-HANSENII; ACTIVATED-CHARCOAL; DETOXIFICATION; GUILLIERMONDII; HYDROLYZATE; ETHANOL; IMMOBILIZATION; BIOCONVERSION; TEMPERATURE;
D O I
10.1016/j.carbpol.2012.11.033
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A maximum xylose extraction of 21.98 g/L was obtained in hydrolysate with a solid to liquid ratio of 1:8 (w/v) at 1% H2SO4 and treated for 30 min. The optimized and treated corncob hemicellulosic hydrolysate medium supplemented with (g/L) yeast extract 5.0, KH2PO4 2.0, MgSO4 center dot 7H(2)O 0.3 and methanol 10 mL whose pH was adjusted to 4.5 acts as production medium. Under this condition; the adapted strain of C. tropicalis resulted in 1.22-fold increase in xylitol yield and 1.70-fold enhancement in volumetric productivity was obtained as compared to parent strain of C. tropicalis. On concentrating the hydrolysate under vacuum using rotavapor proves to be efficient in terms of improved xylitol yield and productivity over microwave assisted concentration using adapted strain of C. tropicalis. The immobilized cells of C. tropicalis resulted in more than 70% efficiency up to third cycle. The xylitol production could be scaled up to 10 L fermentor. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1596 / 1601
页数:6
相关论文
共 46 条
[1]  
BUCKE C, 1987, METHOD ENZYMOL, V135, P175
[2]   Clarification of a wheat straw-derived medium with ion-exchange resins for xylitol crystallization [J].
Canilha, Larissa ;
Carvalho, Walter ;
Giulietti, Marco ;
Gracas Almeida Felipe, Maria Das ;
Silva, Joao Batista Almeida E. .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (05) :715-721
[3]   Evaluation of the detoxification of brewery's spent grain hydrolysate for xylitol production by Debaryomyces hansenii CCMI 941 [J].
Carvalheiro, F ;
Duarte, LC ;
Lopes, S ;
Parajó, JC ;
Pereira, H ;
Gírio, FM .
PROCESS BIOCHEMISTRY, 2005, 40 (3-4) :1215-1223
[4]   A study on xylitol production from sugarcane bagasse hemicellulosic hydrolysate by Ca-alginate entrapped cells in a stirred tank reactor [J].
Carvalho, W ;
Santos, JC ;
Canilha, L ;
Silva, JBAE ;
Felipe, MGA ;
Mancilha, IM ;
Silva, SS .
PROCESS BIOCHEMISTRY, 2004, 39 (12) :2135-2141
[5]  
Carvalho W, 2002, Z NATURFORSCH C, V57, P109
[6]   Bioconversion of novel substrate Saccharum spontaneum, a weedy material, into ethanol by Pichia stipitis NCIM3498 [J].
Chandel, Anuj K. ;
Singh, Om V. ;
Rao, L. Venkateswar ;
Chandrasekhar, G. ;
Narasu, M. Lakshmi .
BIORESOURCE TECHNOLOGY, 2011, 102 (02) :1709-1714
[7]   Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'Biofuel' [J].
Chandel, Anuj K. ;
Singh, Om V. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 89 (05) :1289-1303
[8]  
Cho C., 2000, J JAPAN SOC WASTE MA, V11, P11
[9]   PVA-Hydrogel Entrapped Candida Guilliermondii for Xylitol Production from Sugarcane Hemicellulose Hydrolysate [J].
da Cunha, Mario A. A. ;
Converti, Attilio ;
Santos, Julio C. ;
Ferreira, Sylvia T. S. ;
da Silva, Silvio S. .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 157 (03) :527-537
[10]  
Ding XH, 2006, APPL BIOCHEM BIOTECH, V133, P263, DOI 10.1385/ABAB:133:3:263