Extracellular Nucleic Acids in Urine: Sources, Structure, Diagnostic Potential

被引：82

作者：

Bryzgunova, O. E. ^{[1
]}

Laktionov, P. P. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia

[2] EN Meshalkin Novosibirsk Res Inst Circulat Pathol, Novosibirsk 630055, Russia

来源：

ACTA NATURAE | 2015年 / 7卷 / 03期

关键词：

urinary cell-free DNA and RNA; apoptosis; necrosis; active secretion; urine nucleases; cell-free NA based non-invasive diagnostics; DNA METHYLATION BIOMARKERS; DEOXYRIBONUCLEASE-I DNASE; PROSTATE-CANCER; CELL-FREE; APOPTOTIC BODIES; MESSENGER-RNA; CIRCULATING DNA; MULTIPLE GENES; TUMOR-MARKERS; BODY-FLUIDS;

D O I：

10.32607/20758251-2015-7-3-48-54

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-free nucleic acids (cfNA) may reach the urine through cell necrosis or apoptosis, active secretion of nucleic acids by healthy and tumor cells of the urinary tract, and transport of circulating nucleic acids (cirNA) from the blood into primary urine. Even though urinary DNA and RNA are fragmented, they can be used to detect marker sequences. MicroRNAs are also of interest as diagnostic probes. The stability of cfNA in the urine is determined by their structure and packaging into supramolecular complexes and by nuclease activity in the urine. This review summarizes current data on the sources of urinary cfNA, their structural features, diagnostic potential and factors affecting their stability.

引用

页码：48 / 54

页数：7

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