Green biosynthesis of ZnO nanoparticles using Vitex negundo L. extract: Spectroscopic investigation of interaction between ZnO nanoparticles and human serum albumin

被引：106

作者：

Ambika, Subramanian ^{[1
]}

Sundrarajan, Mahalingam ^{[1
]}

机构：

[1] Alagappa Univ, Sch Chem Sci, Dept Ind Chem, Adv Green Organ Chem Lab, Karaikkudi 630003, Tamil Nadu, India

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2015年 / 149卷

关键词：

Green synthesis; ZnO NPs; Human serum albumin; Fluorescence quenching; Circular dichroism; ZINC-OXIDE NANOPARTICLES; SILVER NANOPARTICLES; FLUORESCENCE SPECTROSCOPY; GOLD NANOPARTICLES; ACID;

D O I：

10.1016/j.jphotobiol.2015.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc oxide nanoparticles are widely used as an important kind of biomaterials. The biocompatibility of nanoparticles is important for specific biomedical applications. In this present investigation, ZnO NPs were synthesized via greener method using Vitex negundo L extract and characterized by XRD, UV-DRS and DLS analysis. The UV-visible absorption and fluorescence spectroscopic studies were performed to investigate the binding interaction between HSA and ZnO NPs. The Stern-Volmer constants (K-sv), quenching rate constants (k(g)), number of binding sites and binding constants were calculated from relevant fluorescence quenching data. Moreverer circular dichroism (CD) spectroscopy used to study the conformational changes of protein in the presence of ZnO NPs. (C) 2015 Elsevier B.V. All rights reserved.

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页码：143 / 148

页数：6

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