Mass Spectral Imaging of Glycophospholipids, Cholesterol, and Glycophorin A in Model Cell Membranes

被引：28
作者：
Baker, Matthew J. [1 ,2 ]
Zheng, Leiliang [2 ]
Winograd, Nicholas [2 ]
Lockyer, Nicholas P. [1 ]
Vickerman, John C. [1 ]
机构：
[1] Univ Manchester, Sch Chem Engn & Analyt Sci, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
[2] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
来源：
LANGMUIR | 2008年 / 24卷 / 20期
基金：
英国工程与自然科学研究理事会; 美国国家科学基金会;
关键词：
D O I：
10.1021/la802582f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/ glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images.
引用
收藏
页码：11803 / 11810
页数：8
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