Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

被引：32
作者：
Lee, Su Jin [1 ]
Park, Jee-Woong [1 ]
Kim, In-Ae [1 ]
Youn, Byung-Soo [2 ]
Gu, Man Bock [1 ]
机构：
[1] Korea Univ, Coll Life Sci & Biotechnol, Seoul 136701, South Korea
[2] Adipogen Inc, Inchon 406840, South Korea
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 168卷
关键词：
Aptarners; Adipokines; Antibodies; ELAAS biosensor; Type; 2; diabetes; VISCERAL ADIPOSE-TISSUE; INSULIN-RESISTANCE; DNA APTAMERS; RETINOL-BINDING-PROTEIN-4; NANOPARTICLES; ASSOCIATION; BIOSENSOR; SELECTION;
D O I：
10.1016/j.snb.2012.04.016
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotina mide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three protein:; were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (S PR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field. (C) 2012 Elsevier B.V. All rights reserved.
引用
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页码：243 / 248
页数：6
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