Identification and characterization of a family of Rab11-interacting proteins

被引:246
作者
Hales, CM
Griner, R
Hobdy-Henderson, KC
Dorn, MC
Hardy, D
Kumar, R
Navarre, J
Chan, EKL
Lapierre, LA
Goldenring, JR
机构
[1] Med Coll Georgia, Inst Mol & Med Genet, Dept Med, Augusta, GA 30912 USA
[2] Med Coll Georgia, Inst Mol & Med Genet, Dept Surg, Augusta, GA 30912 USA
[3] Med Coll Georgia, Inst Mol & Med Genet, Dept Cellular Biol & Anat, Augusta, GA 30912 USA
[4] Augusta Vet Affairs Med Ctr, Augusta, GA 30912 USA
[5] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
关键词
D O I
10.1074/jbc.M104831200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha -helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rab11a and H+K+-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rab11a and the H+K+-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rab11a in plasma membrane recycling systems is dependent upon a compendium of protein effectors.
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收藏
页码:39067 / 39075
页数:9
相关论文
共 42 条
[1]   RECEPTOR-MEDIATED TRANSCYTOSIS OF IGA IN MDCK CELLS IS VIA APICAL RECYCLING ENDOSOMES [J].
APODACA, G ;
KATZ, LA ;
MOSTOV, KE .
JOURNAL OF CELL BIOLOGY, 1994, 125 (01) :67-86
[2]   REDISTRIBUTION OF 23 KDA TUBULOVESICLE-ASSOCIATED GTP-BINDING PROTEINS DURING PARIETAL-CELL STIMULATION [J].
BASSON, MD ;
GOLDENRING, JR ;
TANG, LH ;
LEWIS, JJ ;
PADFIELD, P ;
JAMIESON, JD ;
MODLIN, IM .
BIOCHEMICAL JOURNAL, 1991, 279 :43-48
[3]   Genomic structure of murine Rab11 family members [J].
Bhartur, SG ;
Calhoun, BC ;
Woodrum, J ;
Kurkjian, J ;
Iyer, S ;
Lai, F ;
Goldenring, JR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 269 (02) :611-617
[4]   THE SMALL GTPASE RAB5 FUNCTIONS AS A REGULATORY FACTOR IN THE EARLY ENDOCYTIC PATHWAY [J].
BUCCI, C ;
PARTON, RG ;
MATHER, IH ;
STUNNENBERG, H ;
SIMONS, K ;
HOFLACK, B ;
ZERIAL, M .
CELL, 1992, 70 (05) :715-728
[5]   Rab11a redistributes to apical secretory canaliculus during stimulation of gastric parietal cells [J].
Calhoun, BC ;
Lapierre, LA ;
Chew, CS ;
Goldenring, JR .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1998, 275 (01) :C163-C170
[6]  
CARR DW, 1992, J BIOL CHEM, V267, P13376
[7]   Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells [J].
Casanova, JE ;
Wang, XY ;
Kumar, R ;
Bhartur, SG ;
Navarre, J ;
Woodrum, JE ;
Altschuler, Y ;
Ray, GS ;
Goldenring, JR .
MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (01) :47-61
[8]   LOCALIZATION OF LOW-MOLECULAR-WEIGHT GTP BINDING-PROTEINS TO EXOCYTIC AND ENDOCYTIC COMPARTMENTS [J].
CHAVRIER, P ;
PARTON, RG ;
HAURI, HP ;
SIMONS, K ;
ZERIAL, M .
CELL, 1990, 62 (02) :317-329
[9]   MOLECULAR-CLONING OF YPT1/SEC4-RELATED CDNAS FROM AN EPITHELIAL-CELL LINE [J].
CHAVRIER, P ;
VINGRON, M ;
SANDER, C ;
SIMONS, K ;
ZERIAL, M .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6578-6585
[10]   THE COMPLEXITY OF THE RAB AND RHO GTP-BINDING PROTEIN SUBFAMILIES REVEALED BY A PCR CLONING APPROACH [J].
CHAVRIER, P ;
SIMONS, K ;
ZERIAL, M .
GENE, 1992, 112 (02) :261-264