Synthesis and characterization of zinc sulfide quantum dots and their interaction with snake gourd (Trichosanthes anguina) seed lectin

被引：13

作者：

Ahmed, Khan Behlol Ayaz ^{[1
]}

Ahalya, Pichaikkannu ^{[1
]}

Sengan, Megarajan ^{[1
]}

Kamlekar, Ravikanth ^{[2
]}

Anbazhagan, Veerappan ^{[1
]}

机构：

[1] SASTRA Univ, Sch Chem & Biotechnol, Dept Chem, Thanjavur, Tamil Nadu, India

[2] Vellore Inst Technol, Sch Adv Sci, Environm & Analyt Chem Div, Vellore 632014, Tamil Nadu, India

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2015年 / 151卷

关键词：

Zinc sulfide quantum dots; Pectin; Lectin; Snake gourd seed lectin; Binding; Thermodynamics; CONCANAVALIN-A; THERMODYNAMIC ANALYSIS; SERUM-ALBUMIN; BINDING-SITE; FLUORESCENCE; PROTEINS; RESIDUES; CONJUGATE; DRUG;

D O I：

10.1016/j.saa.2015.07.035

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Owing to the use of quantum dots in biological labeling, and the specific interaction of lectins with tumor cells, studies on lectin-QDs interaction are of potential interest. Herein, we report a facile method to prepare zinc sulfide quantum dots (ZnS QDs) using pectin as a capping agent and studied their interaction with snake gourd seed lectin (SGSL) by fluorescence spectroscopy. The QDs were characterized by X-ray diffraction, high-resolution transmission electron microscopy, UV-Vis absorption and fluorescence spectroscopy. The thermodynamic forces governing the interaction between ZnS-QDs and SGSL have been delineated from the temperature dependent association constant. These results suggest that the binding between ZnS QDs and SGSL is governed by enthalpic forces with negative entropic contribution. The red shift of synchronous fluorescence spectra showed that the microenvironment around the tryptophan residues of SGSL was perturbed by ZnS-QDs. The obtained results suggest that the development of optical bioimaging agents by using the conjugated lectin-QDs would be possible to diagnose cancerous tissues. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：739 / 745

页数：7

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