Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

被引：53

作者：

Kim, CK

Chung, JD

Park, SH

Burrell, AM

Kamo, KK

Byrne, DH ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA

[2] Sangju Natl Univ, Dept Hort, Sangju 742711, South Korea

[3] Kyungpook Natl Univ, Dept Hort, Taegu 702701, South Korea

[4] ARS, USDA, Florist & Nursery Plants Res Unit, Beltsville, MD 20705 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 78卷 / 02期

关键词：

genetic engineering; marker gene; rose; transgenic plants; vir gene;

D O I：

10.1023/B:TICU.0000022529.16697.90

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.

引用

页码：107 / 111

页数：5

共 50 条

[41] In vitro regeneration and Agrobacterium tumefaciens-mediated genetic transformation of Parkia timoriana (DC.) Merr.: a multipurpose tree legume
Thangjam, Robert
Sahoo, Lingaraj
ACTA PHYSIOLOGIAE PLANTARUM, 2012, 34 (03) : 1207 - 1215
[42] An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation
Sharma, KK
Anjaiah, V
PLANT SCIENCE, 2000, 159 (01) : 7 - 19
[43] Production of herbicide-resistant sweetpotato (Ipomoea batatas (L.) Lam.) plants by Agrobacterium tumefaciens-mediated transformation
Otani, M
Wakita, Y
Shimada, T
BREEDING SCIENCE, 2003, 53 (02) : 145 - 148
[44] Setting up Agrobacterium tumefaciens-mediated transformation of the tropical legume Aeschynomene evenia, a powerful tool for studying gene function in Nod Factor-independent symbiosis
Tisseyre, Pierre
Cartieaux, Fabienne
Chabrillange, Nathalie
Gully, Djamel
Hocher, Valerie
Svistoonoff, Sergio
Gherbi, Hassen
PLOS ONE, 2024, 19 (04):
[45] Factors involved in Agrobacterium tumefaciens-mediated gene transfer into Pinus nigra Arn. ssp salzmannii (Dunal) Franco
López, M
Humara, JM
Rodríguez, R
Ordás, RJ
EUPHYTICA, 2000, 114 (03) : 195 - 203
[46] Agrobacterium tumefaciens -: mediated transformation of blackgram:: an assessment of factors influencing the efficiency of uidA gene transfer
Saini, R.
Jaiwal, P. K.
BIOLOGIA PLANTARUM, 2007, 51 (01) : 69 - 74
[47] Comprehensive screening of influential factors in the Agrobacterium tumefaciens-mediated transformation of the Himalayan elixir: Ajuga bracteosa Wall. ex. Benth
Kayani, Waqas Khan
Fattahi, Mohammad
Palazon, Javier
Cusido, Rosa M.
Mirza, Bushra
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS, 2016, 3 (04) : 151 - 159
[48] A high-throughput Agrobacterium tumefaciens-mediated transformation system for molecular breeding and functional genomics of rice (Oryza sativa L.)
Shrawat, Ashok K.
Good, Allen G.
PLANT BIOTECHNOLOGY, 2010, 27 (01) : 47 - 58
[49] Agrobacterium tumefaciens — mediated Transformation of Rice Using Coleoptile and Mature Seed-derived Callus
Tarun Kant
S. L. Kothari
Halina Kononowicz-Hodges
Thomas K. Hodges
Journal of Plant Biochemistry and Biotechnology, 2001, 10 : 121 - 126
[50] Evaluation of transformation in peach Prunus persica explants using green fluorescent protein (GFP) and beta-glucuronidase (GUS) reporter genes
Padilla, IMG
Golis, A
Gentile, A
Damiano, C
Scorza, R
PLANT CELL TISSUE AND ORGAN CULTURE, 2006, 84 (03) : 309 - 314

← 1 2 3 4 5 →