Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

被引：53
|
作者：
Kim, CK
Chung, JD
Park, SH
Burrell, AM
Kamo, KK
Byrne, DH [1 ]
机构：
[1] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA
[2] Sangju Natl Univ, Dept Hort, Sangju 742711, South Korea
[3] Kyungpook Natl Univ, Dept Hort, Taegu 702701, South Korea
[4] ARS, USDA, Florist & Nursery Plants Res Unit, Beltsville, MD 20705 USA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 78卷 / 02期
关键词：
genetic engineering; marker gene; rose; transgenic plants; vir gene;
D O I：
10.1023/B:TICU.0000022529.16697.90
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.
引用
收藏
页码：107 / 111
页数：5
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