Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes

被引：32

作者：

Acosta, Hector ^{[1
]}

Burchmore, Richard ^{[2
]}

Naula, Christina ^{[2
]}

Gualdron-Lopez, Melisa ^{[3
,4
]}

Quintero-Troconis, Ender ^{[1
]}

Caceres, Ana J. ^{[1
]}

Michels, Paul A. M. ^{[5
,6
]}

Luis Concepcion, Juan ^{[1
]}

Quinones, Wilfredo ^{[1
]}

机构：

[1] Univ Los Andes, Lab Enzimol Parasitos, Fac Ciencias, Merida 5101, Venezuela

[2] Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[3] Univ Barcelona, Inst Salud Global, Hosp Clin, Barcelona, Spain

[4] Inst Hlth Sci Tries & Pujol, Barcelona, Spain

[5] Univ Edinburgh, Ctr Immun Infect & Evolut, Edinburgh ELF9 3FL, Midlothian, Scotland

[6] Univ Edinburgh, Ctr Translat & Chem Biol, Edinburgh ELF9 3FL, Midlothian, Scotland

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2019年 / 229卷

基金：

英国惠康基金;

关键词：

Trypanosoma cruzi; Glycosome; Glycolysis; Metabolism; Proteomics; Cell fractionation; Mass spectrometry; BLOOD-STREAM-FORM; RAT-LIVER PEROXISOMES; PHOSPHOGLYCERATE KINASE ISOENZYMES; ALPHA-HYDROXYACID DEHYDROGENASE; CONTAINING SUPEROXIDE-DISMUTASE; PYRUVATE PHOSPHATE DIKINASE; ACID BETA-OXIDATION; SUBCELLULAR-LOCALIZATION; BIOCHEMICAL-CHARACTERIZATION; TRYPANOTHIONE REDUCTASE;

D O I：

10.1016/j.molbiopara.2019.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmenta. lized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as beta-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane protein; by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new corn ponents of this parasite's glycosomes.

引用

页码：62 / 74

页数：13

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