Sensitive electrochemical detection of hydroxyl radical based on MBs-DNA-AgNPs nanocomposite

被引：24

作者：

Yang, Yan ^{[1
]}

Zhou, Jiawan ^{[1
]}

Zhang, Yunsong ^{[1
]}

Zou, Qiong ^{[1
]}

Zhang, Xiaohua ^{[1
]}

Chen, Jinhua ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2013年 / 182卷

关键词：

Hydroxyl radicals; DNA damage; Magnetic separation; Ag nanoparticles; Anodic stripping voltammetry; OXIDATIVE DAMAGE; NANOPARTICLES; HYBRIDIZATION; IMMUNOASSAY; BIOSENSORS; CLEAVAGE;

D O I：

10.1016/j.snb.2013.03.071

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive electrochemical method for the detection of hydroxyl radical ((OH)-O-center dot) was successfully developed based on magnetic beads (MBs)-DNA-Ag nanoparticles (AgNPs) (MBs-DNA-AgNPs) nanocomposite. MBs were utilized for bioseparation and AgNPs as signal indicator. When (OH)-O-center dot broke the DNA strands, AgNPs were detached from the surface of the MBs-DNA-AgNPs nanocomposite. Magnetic particles could be easily removed from the solution and the detached AgNPs were collected.Therefore, the anodic stripping voltammetry (ASV) signal of dissolved silver on glassy carbon (GC) electrode depended on the amounts of (OH)-O-center dot. The results show that the ASV signal linearly increases with the increase of the concentration of (OH)-O-center dot in the range of 0.05-4 mu M, and the detection limit is as low as 10 nM, which is much lower than the detection limit obtained on the recently developed electrochemical and photoelectrochemical methods. In addition, the developed method has potential application in the screening of antioxidants. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：504 / 509

页数：6

共 36 条

[1] An electrochemical biosensor for analysis of Fenton-mediated oxidative damage to BSA using poly-o-phenylenediamine as electroactive probe [J].