The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane

被引：42
|
作者：
Aker, Jose
Borst, Jan Willem
Karlova, Rumyana
de Vries, Sacco
机构：
[1] Wageningen Univ, Biochem Lab, NL-6703 HA Wageningen, Netherlands
[2] Wageningen Univ, Microspect Ctr, NL-6703 HA Wageningen, Netherlands
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 2006年 / 156卷 / 01期
关键词：
SERK1; CDC48A; FRET-FLIM; endocytosis;
D O I：
10.1016/j.jsb.2006.03.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescent cell division cycle (CDC)48 proteins were Studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase I (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Forster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK I. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK I in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：62 / 71
页数：10
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