Inner filter effect-modulated ratiometric fluorescence aptasensor based on competition strategy for zearalenone detection in cereal crops: Using mitoxantrone as quencher of CdTe QDs@SiO2

Herein, an innovative ratiometric fluorescence (FL) aptasensor was successfully fabricated for the accurate analysis of zearalenone (ZEN) in corn and barley flour. The ZEN aptamer-modified nitrogen doped graphene quantum dots (NGQDs-apt) and silica sphere-encapsulated cadmium telluride quantum dots (CdTe QDs@SiO2) were directly mixed and applied as ratiometric probes. In the absence of ZEN, mitoxantrone (MTX), which was innovatively introduced as quencher, was captured by NGQDs-apt and its inner filter effect (IFE) on CdTe QDs@SiO2 was inhibited. When ZEN existed, MTX separated from NGQDs-apt and re-dispersed around CdTe QDs@SiO2 owing to the competitive binding of ZEN with its aptamer. As the IFE of free MTX on CdTe QDs@SiO2 recovering, the FL intensity of CdTe QDs@SiO2 was quenched, while the FL intensity of NGQDs-apt was nearly invariant. On this basis, a ratiometric FL aptasensor for ZEN was fabricated, which exhibited outstanding detection performances with a desirable detection limit of 0.32 pg mL(-1).