Pretreatment of low-abundance peptides on detonation nanodiamond for direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引:15
作者
Wei, Li-Ming [1 ,2 ]
Shen, Qian [3 ]
Lu, Hao-Jie [1 ,2 ]
Yang, Peng-Yuan [1 ,2 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
[3] Fudan Univ, Renal Dept Childrens Hosp, Shanghai 200032, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 29期
基金
中国国家自然科学基金;
关键词
Detonation nanodiamond; Sample pretreatment; Low-abundance peptides; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; MAGNETIC SILICA MICROSPHERES; SOLID-PHASE EXTRACTION; TOF-MS; PROTEOME ANALYSIS; CARBON NANOTUBES; ENRICHMENT; MALDI; PROTEINS; NANOPARTICLES; PHOSPHOPEPTIDES;
D O I
10.1016/j.jchromb.2009.09.004
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detonation nanodiamond (dND) was firstly employed as adsorbent for pretreatment of peptides in dilute/contaminated sample solution. Detonation nanodiamond showed high efficiency for isolating and enriching peptides in a wide pH range. Remarkably, good tolerance capability toward salts and detergents could be achieved by using dNDs. Due to the inherent specificities of dNDs, dND-bound peptides could be directly analyzed by MALDI-TOF MS, so as to avoid the elution step and reduce sample loss. This pretreatment method also exhibited a better performance for protein identification compared to solvent evaporation and Ziptip pretreatment approach. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:3631 / 3637
页数:7
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