Pretreatment of low-abundance peptides on detonation nanodiamond for direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
被引:15
作者:
Wei, Li-Ming
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机构:
Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R ChinaFudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Wei, Li-Ming
[1
,2
]
Shen, Qian
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机构:
Fudan Univ, Renal Dept Childrens Hosp, Shanghai 200032, Peoples R ChinaFudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Shen, Qian
[3
]
Lu, Hao-Jie
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机构:
Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R ChinaFudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Lu, Hao-Jie
[1
,2
]
Yang, Peng-Yuan
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R ChinaFudan Univ, Dept Chem, Shanghai 200032, Peoples R China
Yang, Peng-Yuan
[1
,2
]
机构:
[1] Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
[3] Fudan Univ, Renal Dept Childrens Hosp, Shanghai 200032, Peoples R China
来源:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2009年
/
877卷
/
29期
Detonation nanodiamond (dND) was firstly employed as adsorbent for pretreatment of peptides in dilute/contaminated sample solution. Detonation nanodiamond showed high efficiency for isolating and enriching peptides in a wide pH range. Remarkably, good tolerance capability toward salts and detergents could be achieved by using dNDs. Due to the inherent specificities of dNDs, dND-bound peptides could be directly analyzed by MALDI-TOF MS, so as to avoid the elution step and reduce sample loss. This pretreatment method also exhibited a better performance for protein identification compared to solvent evaporation and Ziptip pretreatment approach. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.