Aptamer-based thin film gold electrode modified with gold nanoparticles and carboxylated multi-walled carbon nanotubes for detecting oxytetracycline in chicken samples

被引：48
作者：
He, Baoshan [1 ]
Wang, Long [1 ]
Dong, Xiaoze [1 ]
Yan, Xiaohai [1 ]
Li, Ming [1 ]
Yan, Sasa [1 ]
Yan, Dandan [1 ]
机构：
[1] Henan Univ Technol, Henan Key Lab Cereal & Oil Food Safety Inspect &, Sch Food Sci & Technol, Lianhua Rd 100, Zhengzhou 450001, Henan, Peoples R China
来源：
FOOD CHEMISTRY | 2019年 / 300卷
基金：
中国国家自然科学基金;
关键词：
Oxytetracycline (OTC); Electrochemical aptasensor; CMWCNTs; AuNPs; DNA BIOSENSOR; TETRACYCLINE; ANTIBIOTICS; APTASENSOR; RESIDUES; SENSOR; ASSAY;
D O I：
10.1016/j.foodchem.2019.125179
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this work, a disposable and portable aptasensor for the fast and sensitive detection of oxytetracycline (OTC) using gold nanoparticles (AuNPs)/carboxylated multi-walled carbon nanotubes (cMWCNTs)@thionine connecting complementary strand of aptamer (cDNA) as signal tags was constructed. The substrate electrode of the aptasensor was thin film gold electrode (TFGE), which have the advantages of portable and uniform performance. In the presence of OTC, OTC competed with cDNA to combine with aptamer. The bioconjugate (AuNPs/cMWCNTs/cDNA@thionine) was released from the TFGE. Thus, the electrochemical signal declined. Under optimized conditions, the aptasensor exhibited good stability, high selectivity and high sensitivity. Furthermore, the developed electrochemical aptamer-based TFGE had a wide dynamic range of 1 x 10(-13)-1 x 10(-5) g mL(-1) for target OTC with a low detection limit of 3.1 x 10(-14 )g mL(-1) and was successfully used for the determination of OTC in chicken sample.
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页数：6
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