High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinized using mineral oil

被引：80
作者：
Lin, Jianghai [1 ,2 ]
Kennedy, Stephen H. [1 ]
Svarovsky, Therese [3 ]
Rogers, Jeffrey [4 ]
Kemnitz, Joseph W. [3 ]
Xu, Anlong [2 ]
Zondervan, Krina T. [1 ,5 ]
机构：
[1] Univ Oxford, Nuffield Dept Obstet & Gynaecol, Oxford, England
[2] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
[3] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI USA
[4] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA
[5] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford, England
来源：
ANALYTICAL BIOCHEMISTRY | 2009年 / 395卷 / 02期
基金：
英国惠康基金;
关键词：
ENDOMETRIOSIS; TISSUES;
D O I：
10.1016/j.ab.2009.08.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Extracting DNA from formalin-fixed and paraffin-embedded (FFPE) tissue remains a challenge, despite numerous attempts to develop a more effective method. Polymerase chain reaction (PCR) success rates with DNA extracted using Current methods remain low. We extracted DNA from 140 long-term archived FFPE samples using a simple but effective deparaffinization method, removing the wax with mineral oil, and a commercially available DNA extraction kit. DNA quality was subsequently tested in a genotyping experiment with 14 microsatellite markers. High-quality DNA was obtained with a mean PCR success rate of 97% (range: 88-100%) across markers. The results suggested that DNA extracted using this novel method is likely to be suitable for genetic studies involving DNA fragments <200 bp. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：265 / 267
页数：3
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