Efficiency of glucose oxidase immobilized on tannin modified NiFe2O4 nanoparticles on decolorization of dye in the Fenton and photo-biocatalytic processes

Immobilized enzymes are significantly studied both environmental and textile industries. In this work, glucose oxidase was immobilized on the tannin coated NiFe2O4 magnetic nanoparticles for decolorization of indigo carmin. The as-synthesized all magnetic nanoparticles were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, UV-vis spectroscopy, Thermogravimetric analysis, Vibrating sample magnetometer and Scanning electron microscopy. The activity of the free and immobilized glucose oxidase in various temperature and pH values was investigated. Finally, the produced immobilized glucose oxidase was applied for decolorization of indigo carmine due to production of H2O2. The decolorization yields of indigo carmine are 98.6% and 37.6% of the glucose oxidase immobilized on tannin modified NiFe2O4 in presence of the both of UV-light and iron(II)chloride for 90 min, respectively. The results exude that the obtained photocatalysts exhibit excellent both photocatalytic efficiency and easy separation from dye solution by an extemal magnet.