Behavior of CAL72 osteoblast-like cells cultured on zirconia ceramics with different surface topographies

被引:88
作者
Baechle, Maria
Butz, Frank
Huebner, Ute
Bakalinis, Emmanouil
Kohal, Ralf J.
机构
[1] Univ Hosp Freiburg, Sch Dent, Dept Prosthodont, D-79106 Freiburg, Germany
[2] Univ Hosp Freiburg, Sch Dent, Dept Oral & Maxillofacial Surg, D-79106 Freiburg, Germany
[3] Univ Texas, Houston Hlth Sci Ctr, Div Periodont, Dept Stomatol,Dent Branch, Houston, TX USA
关键词
CAL72; cells; image analyzing; proliferation; surface topography; zirconia ceramics;
D O I
10.1111/j.1600-0501.2006.01292.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Because of its inherent strength, biocompatibility, and tooth-like color, zirconia ceramics have the potential to become an alternative to titanium as dental implant material. This study aimed at investigating the osteoblastic response to yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) with different surface topographies. CAL72 osteoblast-like cells were cultured on machined (TZP-m), airborne particle abraded (TZP-s), and airborne particle abraded and acid-etched Y-TZP (TZP-sa) surfaces. Polystyrene and airborne particle abraded with large grit and acid-etched (SLA) titanium served as a reference control. The surface topography was examined by scanning electron microscopy (SEM) and profilometry. At culture days 3, 6, and 12, cell proliferation, at day 12 cell morphology, and cell-covered surface area were determined. The surface roughness of Y-TZP was increased by airborne particle abrasion and additionally by acid etching. No statistically significant differences were found between average roughness (R-a) and maximum peak-to-valley height (Rp-v) values of airborne particle abraded and acid-etched Y-TZP and SLA titanium. Whereas the cell proliferation assay revealed statistically significant greater values at day 3 for surface-treated Y-TZP and polystyrene cultures as compared with machined Y-TZP, no differences between the Y-TZP groups, SLA titanium, and polystyrene were observed at culture days 6 and 12. Cell morphology and cell-covered surface area were not affected by the type of substrate. The results suggest that roughened Y-TZP is an appropriate substrate for the proliferation and spreading of osteoblastic cells.
引用
收藏
页码:53 / 59
页数:7
相关论文
共 52 条
[41]  
2
[42]  
Schliephake H, 1993, Int J Oral Maxillofac Implants, V8, P502
[43]   THE REACTIONS OF BONE, CONNECTIVE-TISSUE, AND EPITHELIUM TO ENDOSTEAL IMPLANTS WITH TITANIUM-SPRAYED SURFACES [J].
SCHROEDER, A ;
VANDERZYPEN, E ;
STICH, H ;
SUTTER, F .
JOURNAL OF MAXILLOFACIAL SURGERY, 1981, 9 (01) :15-25
[44]   Bone tissue responses to surface-modified zirconia implants: A histomorphometric and removal torque study in the rabbit [J].
Sennerby, L ;
Dasmah, A ;
Larsson, B ;
Iverhed, M .
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, 2005, 7 :S13-S20
[45]   A case of allergic reaction to surgical metal clips inserted for postoperative boost irradiation in a patient undergoing breast-conserving therapy [J].
Tamai K. ;
Mitsumori M. ;
Fujishiro S. ;
Kokubo M. ;
Ooya N. ;
Nagata Y. ;
Sasai K. ;
Hiraoka M. ;
Inamoto T. .
Breast Cancer, 2001, 8 (1) :90-92
[46]  
Tinschert J, 2001, OPER DENT, V26, P367
[47]  
Tschernitschek H, 2005, QUINTESSENCE INT, V36, P523
[48]   Dissemination of wear particles to the liver, spleen, and abdominal lymph nodes of patients with hip or knee replacement [J].
Urban, RM ;
Jacobs, JJ ;
Tomlinson, MJ ;
Gavrilovic, J ;
Black, J ;
Peoc'h, M .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2000, 82A (04) :457-477
[49]  
Valentine-Thon E, 2003, NEUROENDOCRINOL LETT, V24, P57
[50]   How to optimize seeding and culturing of human osteoblast-like cells on various biomaterials [J].
Wiedmann-Al-Ahmad, M ;
Gutwald, R ;
Lauer, G ;
Hübner, U ;
Schmelzeisen, R .
BIOMATERIALS, 2002, 23 (16) :3319-3328