Helicobacter pylori induces interleukin-8 expression in endothelial cells and the signal pathway is protein tyrosine kinase dependent

Helicobacter pylori (HP) infection has been shown to increase gastric mucosal interleukin 8 (IL-8) expression, and whether HP or its toxin induces endothelial cell IL-8 expression is unknown. We aimed to compare the IL-8 expression in endothelial cells after stimulation with HP toxin, tumor necrosis factor alpha (TNF-alpha), and lipopolysaccharide (LPS) and to study their signal pathways. HP or its toxin induced significant IL-8 expression in endothelial cells. KP toxin, TNF-alpha, and LPS also showed a time-and dose-dependent increase in IL-8 expression overt the control. Both protein kinase C (PKC) and protein kinase A (PRA) inhibitors had no effect on IL-8 response to these stimuli. Protein tyrosine kinase (PTR) inhibitor genistein at concentrations of 150, 300, and 450 mu M dose-dependently reduced LPS-and TNF-alpha-induced IL-8 expression by 29.43, 43.8, and 47.3% and 20.5, 49.9, and 61.8% respectively, whereas HP toxin-induced IL-8 secretion could only be reduced at 450 mu M by 35.7%. Geldanamycin, a more potent PTR inhibitor, at doses of 0.5, 1, and 2 mu M dose-dependently reduced HP toxin induced endothelial cell IL-8 expression by 24.8, 26, and 44.3% respectively. It is concluded that HP and its toxin can increase IL-8 expression in endothelial cells, and the expression of IL-8 elicited by HP toxin, TNF-alpha, and LPS is partially dependent on PTK but not PKA or PKC activation. (C) 1997 Academic Press.