Inhibitory Effect of n-butanol Fraction of Moringa oleifera Lam. Seeds on Ovalbumin-Induced Airway Inflammation in a Guinea Pig Model of Asthma

被引:43
作者
Mahajan, Shailaja G. [1 ]
Banerjee, Aryamitra [2 ]
Chauhan, Bhupendrasinh F. [2 ]
Padh, Harish [2 ]
Nivsarkar, Manish [2 ]
Mehta, Anita A. [1 ]
机构
[1] LM Coll Pharm, Dept Pharmacol, Ahmadabad 380009, Gujarat, India
[2] BV Patel Pharmaceut Educ & Res Dev Ctr, Ahmadabad, Gujarat, India
关键词
Moringa oleifera; asthma; ovalbumin; cytokines; MAST-CELLS; HISTAMINE; CYTOKINES; EXTRACT; INTERLEUKIN-4; EOSINOPHILS; RESPONSES; RELEASE; ONSET;
D O I
10.1177/1091581809345165
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with aerosolized 0.5% ovalbumin. During the experimental period, bronchoconstriction tests are performed, and lung function parameters are measured. The blood and bronchoalveolar lavage fluid are collected to assess cellular content, and serum is used for cytokine (tumor necrosis factor-alpha, interleukin-4, and interleukin-6) assays. Histamine assays of lung tissue are performed using lung tissue homogenate. The results suggest that in ovalbumin-sensitized model control animals, tidal volume is decreased, respiration rate is increased, and both the total and differential cell counts in blood and bronchoalveolar lavage fluid are increased significantly compared with nonsensitized controls. MONB treatment shows improvement in all parameters except bronchoalveolar lavage tumor necrosis factor-alpha and interleukin-4. Moreover, MONB treatment demonstrates protection against acetylcholine-induced bronchoconstriction and airway inflammation. These results indicate that MONB has an inhibitory effect on airway inflammation. Thus, MONB possesses an antiasthmatic property through modulation of the relationship between Th1/Th2 cytokine imbalances.
引用
收藏
页码:519 / 527
页数:9
相关论文
共 55 条
[21]   THE ONSET AND RECOVERY FROM AIRWAY HYPERRESPONSIVENESS - RELATIONSHIP WITH INFLAMMATORY CELL INFILTRATES AND RELEASE OF CYTOTOXIC GRANULE PROTEINS [J].
GUNDEL, RH ;
WEGNER, CD ;
LETTS, LG .
CLINICAL AND EXPERIMENTAL ALLERGY, 1992, 22 (02) :303-308
[22]  
Hattori Takeshi, 2006, Arerugi, V55, P647
[23]   MEASUREMENT OF PLASMA HISTAMINE IN ASTHMA [J].
IND, PW ;
BARNES, PJ ;
BROWN, MJ ;
CAUSON, R ;
DOLLERY, CT .
CLINICAL ALLERGY, 1983, 13 (01) :61-67
[24]   Effect of isolation on classical conditioned histamine release in guinea pigs [J].
Irie, M ;
Nagata, S ;
Endo, Y .
NEUROSCIENCE RESEARCH, 2002, 44 (01) :31-35
[25]  
KAIK G, 1986, WIEN MED WOCHENSCHR, V136, P637
[26]  
Kirtikar KR., 1975, Indian Med cmal Plants, V1, P676
[27]   Antioxidant capacities and phenolics levels of French wines from different varieties and vintages [J].
Landrault, N ;
Poucheret, P ;
Ravel, P ;
Gasc, F ;
Cros, G ;
Teissedre, PL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (07) :3341-3348
[28]   IMMEDIATE AND LATE INFLAMMATORY RESPONSES TO RAGWEED ANTIGEN CHALLENGE OF THE PERIPHERAL AIRWAYS IN ALLERGIC ASTHMATICS - CELLULAR, MEDIATOR, AND PERMEABILITY CHANGES [J].
LIU, MC ;
HUBBARD, WC ;
PROUD, D ;
STEALEY, BA ;
GALLI, SJ ;
KAGEYSOBOTKA, A ;
BLEECKER, ER ;
LICHTENSTEIN, LM .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (01) :51-58
[29]  
Mahajan SG, 2008, INHAL TOXICOL, V20, P897, DOI [10.1080/08958370802027443, 10.1080/08958370802027443 ]
[30]   Effect of Moringa oleifera Lam. seed extract on toulene diisocyanate-induced immune-mediated inflammatory responses in rats [J].
Mahajan, Shailaja G. ;
Mali, Ravindra G. ;
Mehta, Anita A. .
JOURNAL OF IMMUNOTOXICOLOGY, 2007, 4 (02) :85-96