All major regions of FtsK are required for resolution of chromosome dimers

被引:15
作者
Boyle, DS [1 ]
Grant, D [1 ]
Draper, GC [1 ]
Donachie, WD [1 ]
机构
[1] Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland
关键词
D O I
10.1128/JB.182.14.4124-4127.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry out dif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites.
引用
收藏
页码:4124 / 4127
页数:4
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