Development of a new colloidal gold immunochromatographic strip for rapid detecting subgroup A of avian leukosis virus using colloidal gold nanoparticles

被引:10
作者
Hu, Weiguo [1 ,2 ]
Yan, Zeyi [1 ,2 ]
Li, Hongmei [1 ,2 ]
Qiu, Jianhua [1 ,2 ]
Zhang, Dandan [1 ,2 ]
Li, Peng [1 ,2 ]
Pan, Yao [1 ,2 ]
Guo, Huijun [1 ,2 ]
机构
[1] Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China
关键词
Colloidal gold nanoparticles; Monoclonal antibody; Polyclonal antibody; Immunochromatographic strip; Subgroup A of avian leukosis virus; MONOCLONAL-ANTIBODIES; MYCOPLASMA-SUIS; IDENTIFICATION; RECOMBINANT; GENOME; ELISA; ASSAY; GP85; P27;
D O I
10.1016/j.bej.2019.04.015
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study focuses on developing a new colloidal gold immunochromatographic strip for the rapid and specific detection of subgroup A of avian leukosis virus (ALV-A) in clinical practice based on immunochemistry responses using colloidal gold nanoparticles, monoclonal antibody (MAb) and polyclonal antibody (PAb). The solution containing colloidal gold particles (Phi 20 nm) is prepared by the reduction of chloroauric acid and sodium citrate. The MAb and PAb are used to target ALV-A as colloidal gold-MAb conjugates and capture antibodies, and their optimum concentrations are 10 mu g/mL and 2.0 mg/mL, respectively. The optimum pH in the reaction system for the colloidal gold-MAb complex is pH 8.3. The specificity results show that the developed strip can detect ALV-A but not ALV-B/J/K. The sensitivity of the strip is 32(similar to)64 TCID50/mL for ALV-A detection and 8(similar to)80 ng/mL for ALV-A gp85 protein. The strip remains stable in storage at 4 degrees C for 180 d and can detect ALV-A in many tissue samples, including blood, cloacal swab, and egg albumin samples as well as cell samples in vitro. Additionally, it does not require any equipment, and its assay time and cost are much less than those of ELISA. Therefore, this strip is convenient, rapid and effective for detecting ALV-A in avian clinical applications.
引用
收藏
页码:16 / 23
页数:8
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