Higher physiopathogenicity by Fasciola gigantica than by the genetically close F-hepatica: experimental long-term follow-up of biochemical markers

被引:58
作者
Adela Valero, M. [1 ]
Dolores Bargues, M. [1 ]
Khoubbane, Messaoud [1 ]
Artigas, Patricio [1 ]
Quesada, Carla [1 ]
Berinde, Lavinia [1 ,2 ]
Ubeira, Florencio M. [3 ]
Mezo, Mercedes [4 ]
Hernandez, Jose L. [5 ]
Agramunt, Veronica H. [1 ]
Mas-Coma, Santiago [1 ]
机构
[1] Univ Valencia, Fac Farm, Dept Parasitol, Ave Vicent Andres Estelles S-N, Valencia 46100, Spain
[2] Univ Med & Pharm Iuliu Hatieganu, Dept Microbiol, Cluj Napoca 400394, Romania
[3] Univ Santiago de Compostela, Fac Farm, Parasitol Lab, Santiago De Compostela 15784, Spain
[4] Inst Galego Calidade Alimentaria Xunta de Galicia, Ctr Invest Agr Mabegondo, Parasitol Lab, Carretera AC 542 Betanzos Meson do Vento,Km 7, Abegondo 15318, A Coruna, Spain
[5] Hosp Cent Defensa Gomez Ulla, Lab Anal Clin, Calle Glorieta del Ejercito 1, Madrid 28047, Spain
关键词
Biochemical parameters; Disease phases; Fasciola gigantica; Fasciola hepatica; Physiopathogenicity; Sheep; SHEEP; INFECTION; LIVER; RESPONSES; INDICATORS; DIAGNOSIS; SECONDARY; DISEASE; GLUCOSE; UPDATE;
D O I
10.1093/trstmh/trv110
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Background: Fascioliasis is caused by Fasciola hepatica and F. gigantica. The latter, always considered secondary in human infection, nowadays appears increasingly involved in Africa and Asia. Unfortunately, little is known about its pathogenicity, mainly due to difficulties in assessing the moment a patient first becomes infected and the differential diagnosis with F. hepatica. Methods: A long-term, 24-week, experimental study comparing F. hepatica and F. giganticawas made for the first time in the same animal model host, Guirra sheep. Serum biochemical parameters of liver damage, serum electrolytes, protein metabolism, plasma proteins, carbohydrate metabolism, hepatic lipid metabolism and inflammation were analysed on a biweekly basis as morbidity indicators. Serum anti-Fasciola IgG, coproantigen and egg shedding were simultaneously followed up. Results: rDNA and mtDNA sequencing and the morphometric study by computer image analysis system (CIAS) showed that fasciolids used fitted standard species characteristics. Results demonstrated that F. gigantica is more pathogenic, given its bigger size and biomass but not due to genetic differences which are few. Fasciola gigantica shows a delayed development of 1-2 weeks regarding both the biliary phase and the beginning of egg shedding, with respective consequences for biochemical modifications in the acute and chronic periods. Conclusions: The higher F. gigantica pathogenicity contrasts with previous studies which only reflected the faster development of F. hepatica observed in short-term experiments.
引用
收藏
页码:55 / 66
页数:12
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