Biotinylated Stealth® magnetoliposomes

被引：31
作者：
Hodenius, M
De Cuyper, M
Desender, L
Müller-Schulte, D
Steigel, A
Lueken, H
机构：
[1] Katholieke Univ Leuven, Interdisciplinary Res Ctr, B-8500 Kortrijk, Belgium
[2] Rhein Westfal TH Aachen, D-52074 Aachen, Germany
[3] Univ Dusseldorf, D-40225 Dusseldorf, Germany
来源：
CHEMISTRY AND PHYSICS OF LIPIDS | 2002年 / 120卷 / 1-2期
关键词：
magnetoliposomes; phospholipid-protein conjugates; phospholipid vesicles; poly(ethylene glycol)-biotin derivatized; phospholipids; streptavidinylated enzymes; Stealth (R) magnetoliposomes;
D O I：
10.1016/S0009-3084(02)00105-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Dimyristoylphosphatidylethanolamine (DC14:0PE) and the dioleoyl analogue (DC18:1cisPE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC14:0PE-PEG-biotin) and the dioleoyl analogue DC18:1cisPE-PEG-biotin, respectively. As shown by thin-layer chromatography and H-1 NMR spectroscopy, PEGylation of both phosphatidylethaholamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(R)(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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收藏
页码：75 / 85
页数：11
相关论文
共 46 条
[1]  
Allen Theresa M., 1994, Journal of Liposome Research, V4, P1, DOI 10.3109/08982109409037027
[2]   A NEW STRATEGY FOR ATTACHMENT OF ANTIBODIES TO STERICALLY STABILIZED LIPOSOMES RESULTING IN EFFICIENT TARGETING TO CANCER-CELLS [J].
ALLEN, TM ;
BRANDEIS, E ;
HANSEN, CB ;
KAO, GY ;
ZALIPSKY, S .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1237 (02) :99-108
[3]   Phospholipid component volumes: Determination and application to bilayer structure calculations [J].
Armen, RS ;
Uitto, OD ;
Feller, SE .
BIOPHYSICAL JOURNAL, 1998, 75 (02) :734-744
[4]  
Baldeschwieler JD, 1997, CHEMTECH, V27, P34
[5]   CONFORMATION OF PROTECTED AMINO-ACIDS .6. TERT-BUTOXYCARBONYL-L-ALPHA-PHENYLALANINE - CRYSTAL-STRUCTURE AND CONFORMATIONAL-CHANGES IN SOLUTION [J].
BATS, JW ;
FUESS, H ;
KESSLER, H ;
SCHUCK, R .
CHEMISCHE BERICHTE-RECUEIL, 1980, 113 (02) :520-530
[6]  
BAYER EA, 1979, METHOD BIOCHEM ANAL, V26, P1
[7]   MOLECULAR MECHANISM OF THE LIPID VESICLE LONGEVITY INVIVO [J].
BLUME, G ;
CEVC, G .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1146 (02) :157-168
[8]   N,N′-disuccinimidyl carbonate as a coupling agent in the synthesis of thiophospholipids used for anchoring biomembranes to gold surfaces [J].
Boden, N ;
Bushby, RJ ;
Liu, QY ;
Evans, SD ;
Jenkins, TA ;
Miles, RE .
TETRAHEDRON, 1998, 54 (38) :11537-11548
[9]   CONFORMATION OF PRESERVED AMINO-ACIDS .1. NMR-STUDIES OF T-BOC-GLYCINE [J].
BRANIK, M ;
KESSLER, H .
TETRAHEDRON, 1974, 30 (06) :781-786
[10]   CONFORMATION OF PROTECTED AMINO-ACIDS .4. NMR AND IR INVESTIGATIONS OF BOC-OMEGA AMINO ACIDS [J].
BRANIK, M ;
KESSLER, H .
CHEMISCHE BERICHTE-RECUEIL, 1975, 108 (08) :2722-2727
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