Large-scale sequencing and comparative analysis of oenological Saccharomyces cerevisiae strains supported by nanopore refinement of key genomes

被引：17

作者：

Basile, Arianna ^{[1
]}

De Pascale, Fabio ^{[1
]}

Bianca, Federico ^{[1
]}

Rossi, Alessandro ^{[1
]}

Frizzarin, Martina ^{[2
,3
]}

De Bernardini, Nicola ^{[1
]}

Bosaro, Matteo ^{[3
]}

Baldisseri, Anna ^{[2
]}

Antoniali, Paolo ^{[3
]}

Lopreiato, Raffaele ^{[2
]}

Treu, Laura ^{[1
]}

Campanaro, Stefano ^{[1
,4
]}

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

[2] Univ Padua, Dept Biomed Sci, I-35131 Padua, Italy

[3] Italiana Biotecnol, Via Vigazzolo 112, I-36054 Montebello Vicentino, Italy

[4] Univ Padua, CRIBI Biotechnol Ctr, I-35121 Padua, Italy

来源：

FOOD MICROBIOLOGY | 2021年 / 97卷

关键词：

Saccharomyces cerevisiae; Oenological strains; Winemaking; Genomic variants; SNP; Comparative genomics;

D O I：

10.1016/j.fm.2021.103753

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Saccharomyces cerevisiae has long been part of human activities related to the production of food and wine. The industrial demand for fermented beverages with well-defined and stable characteristics boosted the isolation and selection of strains conferring a distinctive aroma profile to the final product. To uncover variants characterizing oenological strains, the sequencing of 65 new S. cerevisiae isolates, and the comparison with other 503 publicly available genomes were performed. A hybrid approach based on short Illumina and long Oxford Nanopore reads allowed the in-depth investigation of eleven genomes and the identification of putative laterally transferred regions and structural variants. A comparative analysis between clusters of strains belonging to different datasets allowed the identification of novel relevant genetic features including single nucleotide polymorphisms, insertions and structural variants. Detection of oenological single nucleotide variants shed light on the existence of different levels of modulation for the mevalonate pathway relevant for the biosynthesis of aromatic compounds.

引用

页数：11

共 93 条

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