Detection of Allergen Walnut Component in Food by an Improved Real-Time PCR Method

被引：15

作者：

Wang, Haiyan ^{[1
,2
]}

Yuan, Fei ^{[1
]}

Wu, Yajun ^{[1
]}

Yang, Hairong ^{[1
]}

Xu, Baoliang ^{[1
]}

Liu, Zhongxue ^{[2
]}

Chen, Ying ^{[1
]}

机构：

[1] Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing 100123, Peoples R China

[2] Inner Mongolia Entry Exit Inspect & Quarantine Bu, Hohhot 010020, Inner Mongolia, Peoples R China

来源：

JOURNAL OF FOOD PROTECTION | 2009年 / 72卷 / 11期

关键词：

TREE NUT ALLERGY; PEANUT; PROTEINS;

D O I：

10.4315/0362-028X-72.11.2433

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A real-time PCR method aimed at the gene sequence of the walnut vicilin-like seed storage protein was established for the detection of the allergen walnut in food. The primers and probe were designed based on published methods. The method provided positive results for walnut and negative results for other tested agricultural plant materials including pecan. The intrinsic detection limit of the method was 0.00125 ng of walnut DNA, and the practical detection limit was 0.001% (wt/wt) walnut content in wheat; both of these values are lower than that of previously published methods. Therefore, this real-time PCR method is sufficiently specific and sensitive for the detection of walnut component in food.

引用

页码：2433 / 2435

页数：3

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