B-Cell Lymphoma-2 Localization in the Female Reproductive Tract of the Chinese Soft-Shelled Turtle, Pelodiscus Sinensis and Its Relationship With Sperm Storage

被引：7
作者：
Chen, Yuan Le Shaofan [1 ]
Hu, Lisi [1 ]
Zhang, Linli [1 ]
Ullah, Shakeeb [1 ]
Liu, Tengfei [1 ]
Yang, Ping [1 ]
Liu, Yi [1 ]
Chen, Qiusheng [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
来源：
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2015年 / 298卷 / 12期
基金：
中国国家自然科学基金;
关键词：
oviduct; Chinese soft-shelled turtle; Bcl-2; Western blot; immunohistochemistry; EPITHELIAL-CELLS; BCL-2; OVIDUCT; ULTRASTRUCTURE; SPERMATOZOA; EXPRESSION; APOPTOSIS; UTERUS;
D O I：
10.1002/ar.23258
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
The aim of the present study was to investigate the expression and localization of B-cell lymphoma-2 (Bcl-2) in the oviduct of the Chinese soft-shelled turtle, Pelodiscus sinensis, during the reproductive cycle to analyze the relationship between Bcl-2 and sperm storage. Bcl-2 expression was confirmed in the P. sinensis oviduct by western blot analysis. Hematoxylin-eosin staining showed that female P. sinensis stored sperm from November to April of the following year. The oviduct showed positive immunostaining for Bcl-2 of epithelial ciliated cells, gland ducts, and gland cells. Bcl-2 expression in the oviduct was associated with sperm storage occurrence. This indicates that the survival factor Bcl-2 may play a role in P. sinensis sperm storage. (C) 2015 Wiley Periodicals, Inc.
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收藏
页码：2011 / 2017
页数：7
相关论文
共 30 条
  • [1] ALDRIDGE RD, 1992, COPEIA, P1103
  • [2] Almeida-Santos Selma Maria, 1997, Japanese Journal of Herpetology, V17, P46
  • [3] Bcl-2 expression is altered with ovarian tumor progression: an immunohistochemical evaluation
    Anderson, Nicole S.
    Turner, Leslie
    Livingston, Sandra
    Chen, Ren
    Nicosia, Santo V.
    Kruk, Patricia A.
    [J]. JOURNAL OF OVARIAN RESEARCH, 2009, 2 (01):
  • [4] The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season
    Bian, Xunguang
    Gandahi, Jameel Ahmed
    Liu, Yue
    Yang, Ping
    Liu, Yi
    Zhang, Linli
    Zhang, Qian
    Chen, Qiusheng
    [J]. MICRON, 2013, 44 : 202 - 209
  • [5] Regulation of mitochondrial morphological dynamics during apoptosis by Bcl-2 family proteins - A key in Bak?
    Brooks, Craig
    Dong, Zheng
    [J]. CELL CYCLE, 2007, 6 (24) : 3043 - 3047
  • [6] Bcl-2 and Bax exert opposing effects on Ca2+ signaling, which do not depend on their putative pore-forming region
    Chami, M
    Prandini, A
    Campanella, M
    Pinton, P
    Szabadkai, G
    Reed, JC
    Rizzuto, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (52) : 54581 - 54589
  • [7] Davenport Michael, 1995, Herpetological Review, V26, P14
  • [8] DENOVO PROTEIN-SYNTHESIS BY BOVINE UTERINE TUBE (OVIDUCT) EPITHELIAL-CELLS CHANGES DURING COCULTURE WITH BULL SPERMATOZOA
    ELLINGTON, JE
    IGNOTZ, GG
    BALL, BA
    MEYERSWALLEN, VN
    CURRIE, WB
    [J]. BIOLOGY OF REPRODUCTION, 1993, 48 (04) : 851 - 856
  • [9] Sperm-induced modification of the oviductal gene expression profile after natural insemination in mice
    Fazeli, A
    Affara, NA
    Hubank, M
    Holt, WV
    [J]. BIOLOGY OF REPRODUCTION, 2004, 71 (01) : 60 - 65
  • [10] Patterns of sperm use in two populations of Red-sided Garter Snake (Thamnophis sirtalis parietalis) with long-term female sperm storage
    Friesen, Christopher R.
    Mason, Robert T.
    Arnold, Stevan J.
    Estes, Suzanne
    [J]. CANADIAN JOURNAL OF ZOOLOGY, 2014, 92 (01) : 33 - 40
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