High-throughput confinement and detection of single DNA molecules in aqueous microdroplets

被引:23
作者
Srisa-Art, Monpichar [1 ]
deMello, Andrew J. [1 ]
Edel, Joshua B. [1 ,2 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England
基金
英国工程与自然科学研究理事会;
关键词
LEVITATED MICRODROPLETS; SPECTROSCOPY; DROPLETS; ASSAYS;
D O I
10.1039/b917721c
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A droplet-based microfluidic system combined with high-sensitivity optical detection is used as a tool for high-throughput confinement and detection of single DNA molecules.
引用
收藏
页码:6548 / 6550
页数:3
相关论文
共 17 条
[1]   High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments [J].
Aharoni, A ;
Amitai, G ;
Bernath, K ;
Magdassi, S ;
Tawfik, DS .
CHEMISTRY & BIOLOGY, 2005, 12 (12) :1281-1289
[2]   DETECTION OF SINGLE RHODAMINE-6G MOLECULES IN LEVITATED MICRODROPLETS [J].
BARNES, MD ;
NG, KC ;
WHITTEN, WB ;
RAMSEY, JM .
ANALYTICAL CHEMISTRY, 1993, 65 (17) :2360-2365
[3]   Single-molecule Spectroscopy using nanoporous membranes [J].
Chansin, Guillaume A. T. ;
Mulero, Rafael ;
Hong, Jongin ;
Kim, Min Jun ;
deMello, Andrew J. ;
Edel, Joshua B. .
NANO LETTERS, 2007, 7 (09) :2901-2906
[4]   Seeing single molecules [J].
de Mello, AJ .
LAB ON A CHIP, 2003, 3 (02) :29N-34N
[5]   Single-molecule fluorescence detection in microfluidic channels -: the Holy Grail in μTAS? [J].
Dittrich, PS ;
Manz, A .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 382 (08) :1771-1782
[6]   Sizing subcellular organelles and nanoparticles confined within aqueous droplete [J].
Gadd, Jennifer C. ;
Kuyper, Christopher L. ;
Fujimoto, Bryant S. ;
Allen, Richard W. ;
Chiu, Daniel T. .
ANALYTICAL CHEMISTRY, 2008, 80 (09) :3450-3457
[7]   Microdroplets: A sea of applications? [J].
Huebner, Ansgar ;
Sharma, Sanjiv ;
Srisa-Art, Monpichar ;
Hollfelder, Florian ;
Edel, Joshua B. ;
deMello, Andrew J. .
LAB ON A CHIP, 2008, 8 (08) :1244-1254
[8]   Development of quantitative cell-based enzyme assays in microdroplets [J].
Huebner, Ansgar ;
Olguin, Luis F. ;
Bratton, Daniel ;
Whyte, Graeme ;
Huck, Wilhelm T. S. ;
de Mello, Andrew J. ;
Edel, Joshua B. ;
Abell, Chris ;
Hollfelder, Florian .
ANALYTICAL CHEMISTRY, 2008, 80 (10) :3890-3896
[9]   Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays [J].
Huebner, Ansgar ;
Bratton, Dan ;
Whyte, Graeme ;
Yang, Min ;
deMello, Andrew J. ;
Abell, Chris ;
Hollfelder, Florian .
LAB ON A CHIP, 2009, 9 (05) :692-698
[10]   Life-on-a-chip [J].
Lidstrom, ME ;
Meldrum, DR .
NATURE REVIEWS MICROBIOLOGY, 2003, 1 (02) :158-164