Mapping of peptides and protein fragments in human urine using liquid chromatography mass spectrometry

被引：64

作者：

Heine, G ^{[1
]}

Raida, M ^{[1
]}

Forssmann, WG ^{[1
]}

机构：

[1] LOWER SAXONY INST PEPTIDE RES,D-30625 HANNOVER,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 776卷 / 01期

关键词：

peptide hormones; peptide sequencing;

D O I：

10.1016/S0021-9673(97)00440-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the mapping of peptide mixtures, heterogeneous with respect to the concentration and the size of individual peptides, was established with the aim of obtaining a comprehensive analysis of human urine peptides. Peptide extraction and fractionation were optimized to achieve a two-step analysis, using reversed-phase and ion-exchange chromatography. Highly sensitive detection of peptides was performed by coupling microbore HPLC with electrospray mass spectrometry (ESI-MS), Peptides such as urodilatin, angiotensin and fragments of psoriasin, granulin and uromodulin were isolated and sequenced. The procedure presented here is a tool for the analysis of complex peptide mixtures from human urine. (C) 1997 Elsevier Science B.V.

引用

收藏

页码：117 / 124

页数：8

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