Isolation and partial characterization of bacteriocin produced from Lactobacillus plantarum

被引：0

作者：

Karthikeyan, V. ^{[1
]}

Santosh, S. W. ^{[1
]}

机构：

[1] SRM Univ, Sch Bioengn, Dept Biotechnol, Kattankulathur 603203, Tamil Nadu, India

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2009年 / 3卷 / 05期

关键词：

Antagonistic activity; bacteriocin; chromatography; Lactobacillus plantarum; PH;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriocin producing Lactobacillus plantarum strain isolated from marine shrimp (Penaeus monodon) gut, showed broad range of antibacterial activity against some major food born pathogens. Maximum bacteriocin production was observed at 50 C, pH 4 and 0.9% sodium chloride solution. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 2.5 KDa based on the plasmid curing experiment, suggesting that the bacteriocin was a plasmid encoded protein molecule. The study revealed the possibility of using bacteriocin as a food preservative and the L. plantarum strain as probiotic.

引用

页码：233 / 239

页数：7

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