INTRANASAL IMMUNIZATION OF MICE WITH VP2 DNA OF HUMAN ROTAVIRUS A INDUCES CELLULAR AND HUMORAL IMMUNITY

被引：0

作者：

Pourasgari, F. ^{[1
]}

Ahmadian, S. ^{[1
]}

Hassan, Z. M. ^{[2
]}

Mahdavi, M. ^{[2
]}

Salmanian, A. H. ^{[3
]}

Sarbolouki, M. N. ^{[1
]}

Pothier, P. ^{[4
,5
]}

机构：

[1] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran

[2] Univ Tarbiat Modarres, Dept Immunol, Tehran, Iran

[3] Natl Inst Genet Engn & Biotechnol, Tehran, Iran

[4] Univ Bourgogne, Fac Med, Virol Lab, Dijon, France

[5] Univ Bourgogne, Fac Med, Ctr Natl Reference Virus Enter, Dijon, France

来源：

ACTA VIROLOGICA | 2008年 / 52卷 / 04期

关键词：

Human rotavirus A; VP2; protein; immunogenicity; DNA vaccine; mouse;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA vaccination using a plasmid encoding Human rotavirus A (HuRV-A) inner capsid VP2 was examined in a mouse model. BALB/c mice were immunized intranasally (i.n.) with a VP2 DNA vaccine that induced cellular and humoral immune response to HuRV-A. The increased levels of cytokines IFN-gamma and IL-4 and the production of anti-VP2 IgG antibodies were detected. In addition, splenocyte proliferation detected by MTT test was enhanced in the mice treated with the vaccine. These results may encourage the development of a HuRV-A DNA vaccine derived from the inner layer of viral capsid that can be administered i.n.

引用

页码：225 / 229

页数：5

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