Metaproteomics: Harnessing the Power of High Performance Mass Spectrometry to Identify the Suite of Proteins That Control Metabolic Activities in Microbial Communities

被引:118
作者
Hettich, Robert L. [1 ]
Pan, Chongle [1 ]
Chourey, Karuna [1 ]
Giannone, Richard J. [1 ]
机构
[1] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA
基金
美国国家卫生研究院;
关键词
SHEWANELLA-ONEIDENSIS MR-1; TOP-DOWN; GEL-ELECTROPHORESIS; SHOTGUN PROTEOMICS; IDENTIFICATION TECHNOLOGY; ENVIRONMENTAL PROTEOMICS; PEPTIDE-IDENTIFICATION; SOIL METAPROTEOMICS; BACTERIAL PHYLA; INTACT PROTEINS;
D O I
10.1021/ac303053e
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The availability of extensive genome information for many different microbes, including unculturable species in mixed communities from environmental samples, has enabled systems-biology interrogation by providing a means to access genomic, transcriptomic, and proteomic information. To this end, metaproteomics exploits the power of high-performance mass spectrometry for extensive characterization of the complete suite of proteins expressed by a microbial community in an environmental sample.
引用
收藏
页码:4203 / 4214
页数:12
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