Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria

被引：12

作者：

Yonezawa, Y ^{[1
]}

Tokunaga, H ^{[1
]}

Ishibashi, M ^{[1
]}

Tokunaga, M ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Agr, Lab Appl & Mol Microbiol, Kagoshima 8900065, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 10期

关键词：

nucleoside diphosphate kinase; halophilic enzyme; eubacterial halophile;

D O I：

10.1271/bbb.65.2343

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl.

引用

页码：2343 / 2346

页数：4

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