Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria

被引：12
|
作者：
Yonezawa, Y [1 ]
Tokunaga, H [1 ]
Ishibashi, M [1 ]
Tokunaga, M [1 ]
机构：
[1] Kagoshima Univ, Fac Agr, Lab Appl & Mol Microbiol, Kagoshima 8900065, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 10期
关键词：
nucleoside diphosphate kinase; halophilic enzyme; eubacterial halophile;
D O I：
10.1271/bbb.65.2343
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl.
引用
收藏
页码：2343 / 2346
页数：4
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