Masking Nanoparticle Surfaces for Sensitive and Selective Colorimetric Detection of Proteins

被引:35
作者
Kim, Byung-Ho [1 ]
Yoon, In Seon [1 ]
Lee, Jae-Seung [1 ]
机构
[1] Korea Univ, Dept Mat Sci & Engn, Seoul 136713, South Korea
基金
新加坡国家研究基金会;
关键词
UNMODIFIED GOLD NANOPARTICLES; CATALYTIC-ACTIVITY; AU NANOPARTICLES; NUCLEIC-ACID; APTAMER; DNA; LYSOZYME; IMMOBILIZATION; ANTIBODIES; REDUCTION;
D O I
10.1021/ac4026169
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have developed a convenient and efficient colorimetric detection system for protein targets using aptamer gold nanoparticle conjugates. We take advantage of the correlation between the catalytic properties and the exposed surface area of the nanoparticles, which is inversely proportional to the amount of the aptamer-bound protein targets. As the concentration of the protein target increases, the nanoparticle surface area becomes more masked, thus increasing the reduction time of 4-nitrophenol for the color change. We also reduce the detection time by either redesigning the aptamer sequences or regulating their density. This detection system is highly selective, discriminating the target protein even at a concentration 1000 times higher than the limit of detection (LOD). Importantly, to the best of our knowledge, the LOD with the unaided eye in this work is the lowest for a colorimetric detection system using lysozyme as a model protein (16 nM). Lysozyme in chicken egg whites is directly analyzed using our detection system, whose results are in excellent agreement with the enzyme-linked immunosorbent assay (ELISA) analysis.
引用
收藏
页码:10542 / 10548
页数:7
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